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International Journal of Food Science and Agriculture

A Review on Influence of Growth Regulator and Culture Condition on Micro-Propagation of Ginger (zingiber officinale)

Author:Tajebe Mosie Date:July 30,2019 Hits:

Abstract

Ginger is used in different medicinal systems of the world for a wide range of disorders. Commercially it is available in various forms, such as green ginger (fresh ginger), dry ginger, ginger powder, ginger oil, ginger oleoresin and preserved ginger. Now a day there is an outbreak of ginger wilt disease throughout the world which pushes researchers and experts to work on specifically;the application of plant tissue culture technique to produce disease free plant has been practiced.  Thus the objective of the study is to review the influence of growth regulator and culture condition on micro propagation of ginger.Culture medium without growth regulator fails to stimulate the shoot bud initiation in the explants after four weeks of culture. Depending up on the type and concentration of growth regulators; number of days required for bud break varies from 6-10. Among the growth regulators, the medium containing BA (2.0-4.0 mgl) induced bud sprout in about 6-7 days. Auxin promotes the growth of intact roots and excised root sections but only at a relatively lower concentration range. If its concentration is high, it will suppress morphogenesis in cultured plants. The interaction of cytokinins and explant type had also significant differences for both leaf number and shoot length. The interaction of BAP and NAA produced high number of shoots (in the range of 14-19 shoots per explants) although BAP alone produced 12-13 shoots and 9 roots per explants.The influences of different growth regulators at different concentrations have important roles in promoting well developed roots. The pH condition of the medium should be adjusted to 5.8 before autoclaving at 1.04 kg/cm2 pressure and 121°C temperature for 15 - 20 minute. All cultures should be incubated in 16 h light/8 h dark photoperiod (cool, white fluorescent light -30μ mol m-2S-1) for successful production of plants.

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A  Review  on  Influence  of  Growth  Regulator  and Culture Condition on Micro-Propagation of Ginger (zingiber officinale)

Tajebe Mosie 
Horticulture department, Holeta Agricultural Research Center, P.O. Box 31, Ethiopia

*Corresponding   author: Tajebe   Mosie, Horticulture department, Holeta Agricultural Research Center, P.O. Box 31, Ethiopia. 
Email: tajebemu@gmail.com

How to cite this paper: Mosie T. (2019) A  Review  on  Influence  of  Growth  Regulator  and Culture Condition on Micro-Propagation of Ginger (zingiber officinale). International Journal of Food Science and Agriculture, 3(3), 196-199. 
DOI: 10.26855/ijfsa.2019.09.009
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