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International Journal of Food Science and Agriculture

A Review on Influence of Growth Regulator and Culture Condition on Micro-Propagation of Ginger (zingiber officinale)

Author:Tajebe Mosie Date:July 30,2019 Hits:


Ginger is used in different medicinal systems of the world for a wide range of disorders. Commercially it is available in various forms, such as green ginger (fresh ginger), dry ginger, ginger powder, ginger oil, ginger oleoresin and preserved ginger. Now a day there is an outbreak of ginger wilt disease throughout the world which pushes researchers and experts to work on specifically;the application of plant tissue culture technique to produce disease free plant has been practiced.  Thus the objective of the study is to review the influence of growth regulator and culture condition on micro propagation of ginger.Culture medium without growth regulator fails to stimulate the shoot bud initiation in the explants after four weeks of culture. Depending up on the type and concentration of growth regulators; number of days required for bud break varies from 6-10. Among the growth regulators, the medium containing BA (2.0-4.0 mgl) induced bud sprout in about 6-7 days. Auxin promotes the growth of intact roots and excised root sections but only at a relatively lower concentration range. If its concentration is high, it will suppress morphogenesis in cultured plants. The interaction of cytokinins and explant type had also significant differences for both leaf number and shoot length. The interaction of BAP and NAA produced high number of shoots (in the range of 14-19 shoots per explants) although BAP alone produced 12-13 shoots and 9 roots per explants.The influences of different growth regulators at different concentrations have important roles in promoting well developed roots. The pH condition of the medium should be adjusted to 5.8 before autoclaving at 1.04 kg/cm2 pressure and 121°C temperature for 15 - 20 minute. All cultures should be incubated in 16 h light/8 h dark photoperiod (cool, white fluorescent light -30μ mol m-2S-1) for successful production of plants.


[1]   Abeykera WKSM, Illeperuma CK, Amunugoda PNRL and Wijeralasm SW. Composition of Ginger (Zingiberofficinale Rosccoe) Clones Dried at Different Temperatures for Oil and Oleoresin Content. Sri Lankan journal of Agricultural sciences. 2005;42:34-42. 

[2]   Ravindran PN and NirmalBabu K. Ginger the Genus Zingiber. United States of America. 2005. 

[3]   Bartley J and Jacobs A. Effects of drying on flavor compounds in Australian-grown ginger (Zingiberofficinale). J. Sci. Food Agric. 2000; 80(2): 209-215. 

[4]   Wresdiyati T, Astawaa M, Muchtadi D and Nardiana Y. Antioxidant Activity of Ginger (Zingiberofficinale Rosccoe) Oleoresin on the Profile of Superoxide Permutase in theKidney of Rats under Stress Condition. Journal Technologi Dan Industri Pangan, 2007; 8(2): 118-122. 

[5]   Food and Agriculture Organization of the United Nations (FAO). Country notes for the FAOSTAT domain on production and prices, www.Faostat.org.2008. 

[6]   Kizhakkayi J and Sasikumar B. Variability for quality traits in a global germplasm collection of ginger (Zingiberofficinale Rosccoe) current trends in biotechnology ginger and pharmacy. 2009; 3(3): 254-259. 

[7]   Guan Z, Guo YH, Sui XL, Li W and Zhang X. Photosynthetica, 2008; 46 (2): 193–201. 

[8]   Das A, Kesari V and Rangan L. Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants. Biol. Plant, 2010; 54:423–429. 

[9]   NirmalBabu K, Samsudeen K, Minoo D, Geetha SPand Ravindran PN. Tissue culture andbiotechnology of Ginger. In: Ravindran, P. N. and NirmalBabu, K. (Eds.). Ginger the Genus Zingiber. 2005. 

[10] Abdelmageed AHA, Faridah QZ, Norhana FMA, Julia AA and Midhzar AK. Micropropagation of Etlingeraelatior (Zingiberaceae) by using axillary bud explants. Journal of Medicinal Plants Research. 2011; 5:4465-4469. 

[11]  Sharma GK, Jagetiya S and Dashora R. General Techniques of Plant Tissue Culture. 2015. 

[12]  upta RK and Verma VS. Micropropagation and hardeningin horticultural crops: Low cost options a case study of Ginger (Zingiberofficinale). In: Proceedings of 11th International Conferenceon Plasticulture and Precision Farming, New Delhi. 2005; 153-56. 

[13]  Kambaska KB and Santilata S. Effect of Plant Growth Regulator on Micropropagtion of Ginger (Zingiberofficinale Rosc.) cv- Suprava and Suruchi. Journal of Agricultural Technology, 2009;5(2): 271-280. 

[14] Edison S, Ramana KV, Sasikumar B, NirmalBabu K andSanthosh JE. Biotechnology of Spices, Medicinal & Aromatic Plants. Indian Society for Spices, Calicut, Kerala, India. 1997. 

[15]  Smith RH. Plant Tissue Culture Techniques and Experiments. United States of America: Academic Press. 2013. 

[16] Biruk Ayenew, Wondyifraw Tefera and Bekele Kassahun. In vitro propagation of Ethiopian ginger (Zingiberofficinale Rosc.) cultivars: Evaluation of explant typesand hormone combinations. African Journal of Biotechnology. 2012; 11(16): 3911-3918. 

[17]  Murashigae T and Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 1962; 15:473-497. 

[18]  Ma X and Gang DR. Phytochemistry, 2006; 67: 2239–2255. 

[19] Wardle K, Dobbs KB and Short KC. In-vitro acclimatization of aseptically cultured plantlets to humidity. Journal of American Society of Horticultural Science, 1983; 108: 386-389. 

[20]  Desjardins YA, Goselin and Yellow S. Acclimatization of In vitro straw berry plantlets in Co2 enriched environment and supplementary lighting. Journal of American Society of Horticultural Science, 1987; 112: 846-852. 

[21] Das A, Kesari V and Rangan L. Micropropagation and cytogenetic assessment of Zingiberspecies of Northeast India. Biotech, 2012;3:471-479. [22]  Resh H, Barbara S. Hydroponic Food Production. 1989.

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A  Review  on  Influence  of  Growth  Regulator  and Culture Condition on Micro-Propagation of Ginger (zingiber officinale)

Tajebe Mosie 
Horticulture department, Holeta Agricultural Research Center, P.O. Box 31, Ethiopia

*Corresponding   author: Tajebe   Mosie, Horticulture department, Holeta Agricultural Research Center, P.O. Box 31, Ethiopia. 
Email: tajebemu@gmail.com

How to cite this paper: Mosie T. (2019) A  Review  on  Influence  of  Growth  Regulator  and Culture Condition on Micro-Propagation of Ginger (zingiber officinale). International Journal of Food Science and Agriculture, 3(3), 196-199. 
DOI: 10.26855/ijfsa.2019.09.009
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