Hill Publishing Group | contact@hillpublisher.com

Hill Publishing Group

Location:Home / Journals / International Journal of Clinical and Experimental Medicine Research /

DOI:http://dx.doi.org/10.26855/ijcemr.2022.07.008

Associations among Lipid Profile, Mucin1 and Cholesterol Ester Transfer Protein Status in Bangladeshi Patients with Cholelithiasis and Effects of Laparoscopic Cholecystectomy on Them

Date: July 18,2022 |Hits: 312 Download PDF How to cite this paper

Abu S. M. Giasuddin1,*, Khadija A. Jhuma2, Mohammad A. M. Chowdhury3, Abu M. M. Haq4

1Professor of Biochemistry & Immunology & Director of Medical Research Unit (MRU), Medical & Health Welfare Trust (MHWT), Medical College for Women and Hospital (MCW&H), Plot-4 Road-8/9 Sector-1, Uttara Model Town, Dhaka-1230, Bangladesh. 

2Professor & Head of Department (HOD) at Department of Biochemistry & Molecular Biology, MCW&H& Member Secretary of MRU, MHWT, Plot-4, Road-8/9, Sector-1, Uttara Model Town, Dhaka-1230, Bangladesh.

3Associate Professor of Surgery, Department of Surgery, MCW&H, MHWT, Plot-4, Road-8/9, Sector-1, Uttara Model Town, Dha-ka-1230, Bangladesh.

4Honorary Professor of Internal Medicine, Department of Medicine, MCW&H and Chairman of MRU, MHWT, Plot-4, Road-8/9, Sector-1, Uttara Model Town, Dhaka-1230, Bangladesh.

*Corresponding author: Abu S. M. Giasuddin

Abstract

Objectives: To evaluate associations among Lipid Profile, Mucin1 CETP status between adult normal control subjects (NCs) and cholelithiasis i.e. gallstone disease (GSD) patients (Pts) preoperatively (I0) and postoperatively (II0) with laparoscopic cholecystectomy. Patients and Methods: Adult Pts with GSD (N=55, Gender: 10 males, 45 females; Age range: 28-60 years, Mean age ± SD: 45.5±12.2 years) and NCs (N: 40, Gender: 16 males, 24 females, Mean age + SD: 42.5+10.5 Years) were those reported in our previous case-control prospective interventional studies carried out from October 2016 to March 2018 at MRU, MHWT, Uttara, Dhaka, Bangladesh. Serum lipid profile (TG, TC, LDL-C, HDL-C), Lp(a), ApoA1, ApoB100, ApoE, Mucin1 and CETP status in NCs (I0) and Pts (I0, II0) were reported in the recent past. Further advanced statistical analyses on these data were made according to the objectives of the present article as stated above regarding associations (correlations) among these staus by using SPSS programme-version 26. Results: About associations among the Lipid Profile and Mucin1 status between NCs (I0) and Pts (I0, II0), significant Pearson correlations were found for Pts-TG (Serum-I0) vs Pts-TG (Serum-II0) (r=0.545, p=0.001), Pts-TC (Serum-I0) vs Pts-TC (Serum-II0) (r=0.385, p=0.025) and Pts-LDL-C (Serum-I0) vs Pts-LDL-C (Serum-II0) (r=0.393, p=0.022). No significant associations (correlations) were obtained for HDL-C among the groups compared (p>0.05). Although significant association (correlation) was obtained for Pts-Mucin1 (Serum-I0) vs Pts-Mucin1 (Serum-II0) (r=0.766, p=0.000), no significant associations (correlations) were found for TG vs Mucin1 status and TC vs Mucin1 status for any of the groups compared (p>0.05). However, significant associations were shown for Pts LDL-C (Serum-I0) vs Pts Mucin1 (Serum-II0) (r=0.381, p=0.026), Pts-HDL-C (Serum-I0) vs Pts-Mucin1 (Serum-II0) (r=0.475, p=0.005) and NCs-HDL-C (Serum-I0) vs Pts-Mucin1 (Bile-I0) (r=0.419, p=0.026) respectively. Regarding associations for TG and CETP status between NCs, Pts (I0) and Pts (II0), no significant correlations were noted for any of the groups compared. About associations for TC levels between NCs and Pts, significant correlation was obtained for Pts-TC (Bile-I0) vs Pts-CETP (Serum-II0) only (r=0.384, p=0.045). Associations for LDL-C vs CETP between NCs and Pts, showed no significant correlations for any of the groups compared (p>0.05). But significant Pearson correlation was obtained for Pts-HDL-C (Bile-I0) vs Pts-CETP (Serum-II0) (r=0.388, p=0.023) only. However, no associations were noted for CETP levels for any of the NCs and Pts groups compared (p>0.05). Conclusions: These results on associations (correlations) among the parameters between NCs and Pts (I0, II0) suggested involvements of TG, TC and LDL-C in the disease process and probable interactions of LDL-C and Mucin1 and HDL-C and CETP in the aetiopathogenesis of the disease. These findings were discussed in the light of recent knowledge available on molecular mechanisms for the pathogenesis of GSD i.e. cholelithiasis.

References

[1] Wang, J., Shen, S., Wang, B., Ni, X., Liu, H., Ni, K., et al. (2020). Serum lipid levels are the risk factors of gallbladder stones: a population-based study in China. Lipids in Health and Disease, 2020, 19(1): 50. https://doi.org/10.1186/s12944-019-1184-3. 

[2] Batajoo, H. and Hazra, N. K. (2013). Analysis of serum lipid profile in Cholelithiasis patients. Journal Nepal Health Research Council, 11 (23): 53-55. DOI: https://doi.org/10.33314/jnhrc.v0i0.345.  

[3] Lemmert, F., Gurusamy, K., Ko, C. W., Miquel, J. F., Mendez-Sanchez, N., Portincasa, P., et al. (2016). Gallstones. Natl Rev Dis Primers, 2016, 2(1): 16024. https://doi.org/10.1038/nrdp.2016.24. No.3. 

[4] Rao, P. J., Jarari, A., ElAwami, H., Patil, T. N. (2012). Lipid profile in bile and serum of cholelithiasis patients – A comparative study. Journal of Basic Medical and Allied Sciences, 1(2): 15-21. 

[5] Ward, H. A., Murphy, N., Weiderpass, E., Leitzmann, M. F., Aglago, E., Gunter, M. J., et al. (2019). Gallstones and incident colorectal cancer in a large pan-European cohort study. International Journal of Cancer, 145(6): 1510-1516. https://doi.org/10.1002/ijc.32090. 

[6] Barahona-Ponce, C., Scherer, D., Brinster, R., Boekstegers, F., Marcelain, K., Garate-Calderon, V., et al. (2021). Gallstones, body mass index, C-reactive protein, and gallbladder cancer: Mendelian randomization analysis of Chilean and European genotype data. Hepatology, 73(5): 1783-1796. https://doi.org/10.1002/hep.31537. 

[7] Wirth, J., di Giuseppe, R., Wientzek, A., Katzke, V. A., Kloss, M., Kaaks, R., et al. (2015). Presence of gallstones and the risk of cardiovascular diseases: the EPIC-Germany cohort study. European Journal of Preventive Cardiology, 22(3): 326-334. https://doi.org/10.1177/2047487313512218. 

[8] Ruhl, C. E. and Everhart, J. E. (2011). Gallstone disease is associated with increased mortality in the United States. Gastroen-terology, 140(2): 508-516. https://doi.org/10.1053/jgastro.2010.10.060. 

[9] Portincasa, P., Di Ciaula, A., Vendemiale, G., Palmieri, V., Moschetta, A., et al. (2001). Gallbladder motility and cholesterol crystallization in bile from patients with pigment and cholesterol gallstones. European Journal of Clinical Investigation, 30: 317-324. https://doi.org/10.1046/j.1365-2362.2000.00639.x.

[10] Malik, A. A., Wani, M. L., Tak, S. I., Irshad, I., Ul-Hasan, N. (2011). Association of dyslipidemiawith cholelithiasis and effect of cholecystectomy on the same. International Journal of Surgery, 9(8): 641-642. https://www.sciencedirector.com.

[11] Skill, N. J., Scott, R. E., Wu, J., Maluffio, M. A. (2011). Hepatocellular carcinoma associated lipid metabolism reprogramming. Journal of Surgical Research, 169(1): 51-56. Doi: https://doi.org/10.1016/j.jsr.2009.09.005.

[12] Hasegawa, T. and Makino, I. (1995). Measurement of apolipoprotein A1 in cholesterol gallstones and gallbladder bile of patients with gallstones. Journal of Gastroenterology, 30(1): 96-102. Doi: https://doi.org/10.1007/BF01211382.

[13] Juvonen, T., Kervinen, K., Kairaluoma, M. I., Kesaniemi, Y. A. (1995). Effect of cholecystectomy on plasma lipid and lipoprotein levels. Hepatogastroenterology, 42(4): 377-382. PMID: 8586372.

[14] Pinheiro-Junior, S., Pinhel, M. A., Nakazone, M. A., Pinheiro, A., Amorim, G. F. S., et al. (2012). Effect of genetic variants related to lipid metabolism as risk factors for cholelithiasis after bariatric surgery in Brazilian population. Obesity Surgery, 22: 623-633.

[15] Everhart, J. E., Khare, M., Hill, M., Maurer, K. R. (1999). Prevalence and ethnic differences in gallbladder disease in the United States. Gastroenterology, 117(3): 632-639. Doi: https://doi.org/10.1016/s0016-5085(99)70456-7.

[16] Amigo, L., Zanlungo, S., Mendoza, H., Miquel, J. F., Nervi, F. (1999). Risk factors and pathogenesis of cholesterol gallstones: state of the Art (Editorial). European Review for Medical and Pharmacological Sciences, 3(6): 241-246.

[17] Avunduk, C. (2008). Gallstones. In: Manual of Gastroenterology: Diagnosis and Therapy, Fourth Edition; London: Lippincott Williams & Wilkins. www.amazon.com/dp/0781769744. 

[18] Smith, B. F. (1987). Human gallbladder mucin binds biliary lipids and promotes cholesterol crystal nucleation in model bile. Journal of lipid Research, 28: 1088-1097.

[19] Davenport, M., Heaton, N., Superina, R. (Editors). Surgery of the Liver, Bile Ducts and Pancreas in Children, 3rd Edition, 2017; London: Taylor & Francis Group. DOI: https://doi.org/10.1201/9781315113791. 

[20] Smith, B. F., LaMont, J. T. (1985). Identification of gallbladder mucin-bilirubin complex in human cholesterol gallstone matrix: effects of reducing agents on in vitro dissolution of matrix and intact gallstones. Journal of Clinical Investigation, 76: 439-445.

[21] Haq, A. M. M., Giasuddin, A. S. M., Jhuma, K. A., Choudhury, M. A. M. (2016). Effect of cholecystectomy on lipid profile in Bangladeshi patients with cholelithiasis. Journal of Metabolic Syndrome, 5(1): 192. Doi: https://doi.org/10.4172/ 2167-0943.100092.

[22] Giasuddin, A. S. M., Jhuma, K. A., Choudhury, M. A. M., Haq, A. M. M. (2016). Lipoprotein (a) status and effect of laparoscopic cholecystectomy on it in Bangladeshi patients with cholelithiasis. Journal of Metabolic Syndrome, 5(4): 216. doi: https://doi.org/10.4172/2167-0943.1000216.

[23] Choudhury, M. A. M., Giasuddin, A. S. M., Jhuma, K. A., Haq, A. M. M. (2018). Effects of laparoscopic cholecystectomy on mucin1 and cholesterol ester transfer protein status in Bangladeshi patients with cholelithiasis. Journal of Metabolic Syndrome, 7(1): 240. Doi: https://doi.org/10.4172/2167-0943.1000240.

[24] Jhuma, K. A., Giasuddin, A. S. M., Choudhury, M. A. M., Haq, A. M. M. (2018). Effects of laparoscopic cholecystectomy on apolipoproteins (ApoA1, ApoB100, ApoE) status in Bangladeshi patients with cholelithiasis. Journal of Clinical & Laboratory Medicine, 3(1): https://doi.org/10.16966/2578-9578.121.

[25] Giasuddin, A. S. M., Jhuma, K. A., Choudhury, M. A. M., Haq, A. M. M. (2021). Associations among lipoprotein(a) and apolipoproteins (A1, B100, E) status in Bangladeshi patients with cholelithiasis and effects of laparoscopic cholecystectomy on the status. American Journal of Biomedical Science & Research, 12(1): 15. AJBSR. MS. ID. 001710. Doi: https://doi.org/10.34297/AJBSR.2021.12.001710.

[26] Zhang, M., Mao, M., Zhang, C., Hu, F., Cui, P., Li, G., et al. (2022). Blood lipid metabolism and the risk of gallstone disease: a multi-center study and meta-analysis. Lipids in Health and Disease, 21: 26. https://doi.org/10.1186/s12944-022-01635-9. 

[27] Osman, A., Ibrahim, A. H., Alzamil, A. M., Alkhalifa, A. M., Badghaish, D. A., Al-Dera, F. H., et al. (2020). Is cholecystectomy in patients with symptomatic uncomplicated cholelithiasis beneficial in improving the lipid profile? Cureus, 12: e6729. https://doi.org/10.7759/cureus.6729. 

[28] Remaley, A. T., Rifai, N., Warnick, G. R. (2015). Lipids, lipoproteins, apolipoproteins and other cardiac risk factors. In: Burtis CA, Bruns DE, Sawyer BG (Editors) Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 7th Edition; St Louis, Missouri, USA: Elsevier; pp. 388-411.

[29] Division, M. H., Ballantyne, C. M., Jacobson, T. A., Ziajka, P. E., Maci, K. C., Dicklin, M. R. (2011). Clinical utility of inflammatory markers and advanced lipoprotein testing: advice from an expert panel of lipid specialists. Journal of Clinical Lipidology, 5(5): 338-367.

[30] Vesper, H. W., Wilson, P. W., Rifai, N. (2012). A massage from the laboratory community to the National Cholesterol Education Program Adult Treatment Panel IV. Clinical Chemistry, 58(3): 523-527. https://doi.org/10.1373/clichem.2011.178202.

[31] Danesh, J., Collins, R., Peto, R. (2000). Lipoprotein (a) and coronary heart disease. Meta-analysis of prospective studies. Circulation, 102(10): 1082-1085. https://doi.org/10.1161/01.CIR.102.10.1082.

[32] Barghash, N. A., Elewa, S. M., Hamdi, E. A., Barghash, A. A., El Dine, R. (2004). Role of plasma homocysteine and lipoprotein (a) in coronary artery disease. British Journal of Biomedical Science, 61(2): 78-83. Doi: https://doi.org/10.1080/09674845.2004.11732648.

[33] Giasuddin, A. S. M., Jhuma, K. A., Mujibul Haq, A. M. (2008). Lipoprotein (a) status in Bangladeshi patients with diabetes mellitus. Journal of the Medical College for Women and Hospital, 6(2): 74-82.

[34] Haq, A. M. M., Giasuddin, A. S. M., Huque, M. M. (2011). Serum total homocysteine and lipoprotein (a) levels in acute myocardial infarction and their response to treatment with vitamins. Journal of the College of Physicians and Surgeons Pakistan, 21(5): 266-270.

[35] Eckel, R. H., Grundy, S. M., Zimmet, P. Z. (2005). The metabolic syndrome. Lancet, 365(9468): 1415-1428. Doi: https://doi.org/10.1016/S0140-6736(05)66378-7.

[36] Mendez-Sanchez, N., Chavez-Tapia, N. C., Motola-Kuba, D., Sanchez-Lara, K., Ponciano-Rodriguez, G., Baptista, H., et al. (2005). Metabolic syndrome as a risk factor for gallstone disease. World Journal of Gastroenterology, 11(11): 1653-1657. Doi: https://doi.org/10.3748/wjg.v11.i11.1653.

[37] Stinton, L. M., Shaffer, E. A. (2012) Epidemiology of gallbladder disease: Cholelithiasis and cancer. Gut and Liver, 6(2): 172-187. Doi: https://doi.org/10.5009/gnl.2012.6.2.172.

[38] Petitti, D. B., Friedman, G. D., Klatsky, A. L. (1981). Association of a history of gallbladder disease with a reduced concentration of high density-lipoprotein cholesterol. The New England Journal of Medicine, 304: 1396-1398. Doi: https://doi.org/10.1056/NEJM198106043042305.

[39] Ahlberg, J. (1979). Serum lipid levels and hyperlipoproteinemia in gallstone patients. Acta Chirurgica Scandinavica, 145: 373-377. https://www.scrip.org.

[40] Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in Adults. (2001). Executive summary of the Third Report of The National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in adults (Adult Treatment Panel III). Journal of the American Medical Association, 285(19): 2486-2497. Doi: https://doi.org/10.1001/jama.285.19.2486.

How to cite this paper

Associations among Lipid Profile, Mucin1 and Cholesterol Ester Transfer Protein Status in Bangladeshi Patients with Cholelithiasis and Effects of Laparoscopic Cholecystectomy on Them

How to cite this paper: Abu S. M. Giasuddin, Khadija A. Jhuma, Mohammad A. M. Chowdhury, Abu M. M. Haq. (2022) Associations among Lipid Profile, Mucin1 and Cholesterol Ester Transfer Protein Status in Bangladeshi Patients with Cholelithiasis and Effects of Laparoscopic Cholecystectomy on Them. International Journal of Clinical and Experimental Medicine Research6(3), 275-285.

DOI: http://dx.doi.org/10.26855/ijcemr.2022.07.008

Volumes & Issues

Free HPG Newsletters

Add your e-mail address to receive free newsletters from Hill Publishing Group.

Contact us

Hill Publishing Group

8825 53rd Ave

Elmhurst, NY 11373, USA

E-mail: contact@hillpublisher.com

Copyright © 2019 Hill Publishing Group Inc. All Rights Reserved.