Identification and Characterization of Antimicrobial peptide

Abstract

The present study and experimentation was conducted to identify and to characterize AMP (antimicrobial peptide) from a plant source. N.sativa’s seeds were used as a source for this study. Protein fractions of seeds were purified by ammonium sulfate precipitation and gel filtration chromatography (GFC). The protein concentration was measured by Bradford assay. Broth assay and antimicrobial assay was done using well plate method against E. coli and S.aureus using all the protein fractions simultaneously. 80% ammonium sulfate cut dialyzed fraction depicted best result showing the presence of antimicrobial peptide. This peptide was further purified from the fraction using GFC and Molecular weight analysis (MW) was done on SDS-PAGE and characterize by antimicrobial activity assay. The seed extract can be pharmaceutically and clinically important antimicrobial agent. Further studies are required to identify the active mechanism of these antimicrobial peptides on microbes.