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OAJRC Environmental Science

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Article Open Access http://dx.doi.org/10.26855/oajrces.2023.06.004

Research on the Influence of Inoculating Bacterium Suspension on Microbial Community Structure in Petroleum Hydrocarbon Contaminated Groundwater

Xueyang Huang1,2, Guo Wei3, Ziting Ding4, Huazhi Wang1, Liping Bai1,*

1Technical Center for Soil, Agriculture and Rural Ecology and Environment, Ministry of Ecology and Environment, Beijing, China.

2School of Water Resources & Environment, China University of Geosciences, Beijing, China.

3Beijing Investment Group Co., Ltd, Beijing, China.

4Shanxi Kunhong Environmental Protection Technology Co., Ltd, Taiyuan, Shanxi, China.

*Corresponding author: Liping Bai

Published: July 31,2023

Abstract

Petroleum hydrocarbon is a common pollutant which has high toxicity and transport property in groundwater. Considering the current supervision demands of groundwater pollution in China, this paper studies the bioremediation method of petroleum hydrocarbon contaminated groundwater. The degradation bacteria were screened and separated through the soil samples collected at a petroleum hydrocarbon contaminated site in Beijing, China. The cultivated bacterial suspension was added by different proportions into the groundwater samples. The influence of different proportions of bacteria suspension on the bacteria community structure in groundwater samples was analyzed, and the bacteria which could use petroleum pollutants as carbon sources and energy sources were studied. The research results showed that addition of bacterial suspension could have significantly effects on the bacterial structure in groundwater sample. Compared with the raw water samples contaminated by petroleum hydrocarbons, the relative abundance of Proteobacteria and Firmicutes phylum level increased respectively from 88% to 97% and 0.4% to 34.2% after 24 hours. The relative abundance of Pseudomonas and Bacillus genus level increased respectively from 67% to 99%, and 0.2% to 32.3% after 24 hours. The advantage bacteria in groundwater samples were considered as petroleum hydrocarbon-related degradation bacteria. The research results could provide theoretical basis for the bioremediation of petroleum contaminated soil and groundwater.

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How to cite this paper

Research on the Influence of Inoculating Bacterium Suspension on Microbial Community Structure in Petroleum Hydrocarbon Contaminated Groundwater

How to cite this paper: Xueyang Huang, Guo Wei, Ziting Ding, Huazhi Wang, Liping Bai. (2023) Research on the Influence of Inoculating Bacterium Suspension on Microbial Community Structure in Petroleum Hydrocarbon Contaminated Groundwater. OAJRC Environmental Science4(1), 22-28.

DOI: http://dx.doi.org/10.26855/oajrces.2023.06.004