International Journal of Food Science and Agriculture

ISSN Print: 2578-3467 Downloads: 122046 Total View: 2263908
Frequency: quarterly ISSN Online: 2578-3475 CODEN: IJFSJ3

Bioaccumulation, Depuration of Heavy Metals (As, Cd, Pb) and Metabolism of These Metals in Body of Mussels (Maretrix Lyrata) During 20 Days in Artificial Media of Culture

Pham Kim Phuong 1,2,*, Nguyễn thị Dung 3, Chu Pham Ngoc Son 4, Lưu Duẩn 1

1 Saigon Technique University—STU of Ho Chi Minh City, Viet Nam.

2 Sai Gon Center of High-Tech Analysis, Viet Nam.

3 Institute of Chemical Technology, Ho Chi Minh City, Viet Nam.

4 Science-Technique Association of Ho Chi Minh City, Viet Nam.

*Corresponding author: Pham Kim Phuong

Published: July 24,2020


The purpose of this work is to try to estimate for the first five days the extent of As, Pb, Cd bioaccumulation by Meretrix Lyrata mussels in artificial media of culture contaminated by heavy metals (As at 1.5 and 2.5 ppm, Pb at 1.5 and 2.5 ppm, Cd at 0.1, 0.5, 1 ppm) and that of release in clean water during the follow-ing 15 days. Quantitation was performed by ICP and AAS with Hydride System. Bioaccumulation increased with heavy metal concentrations in water and in the oder Pb > Cd > As. Release was also observed to increase with the amounts of absorbed metals. After 20 days of experiments, following figures of metal release were obtained (As~100%, Pb~68.9%, and Cd~39.65%). In the case of Cd contamination, no mussel survived after 10 days of experiments even at low Cd concentration of 0.1ppm. Residue of heavy metals in the body of mussels was metabolited to another chemical form. Inogranic Cd was metabolited to Cd-metallothionein and detected by LC/MS ESI(+). As was metabolited to mo-nomethyl arsonic acid (MMA), dimethylarsinic acid (DMA) and detected by HPLC-UV-AAS-HG, and Pb was metabolited to photsphat hydroxyt lead [Pb5(OH)(PO4)3]—which was detected by XRD.


[1] Reinhard Dallinger. (1994). “Invertebrate Organisms as Biological Indicators of heavy metals pollution”, Applied Biochemistry and Biotechnology, Vol 48.

[2] Ritta Cornelis, Xinrong Zhang Louis Mees Jutte Molin Christensen. (1998). “Speciation measurement by HPLC-UV-HG-AAS of dimethylasenic acid and asenobetain in tree candidate lyophilized urine reference materials”, Clinical Chemistry, 48, pp. 92-101.

[3] Regoli F, Orlando E. (1994). “Bioavailability of Biologically Detoxified Lead: Risks Arising from Consumption of Polluted Mussels”, Environ Health Perspect, 102 (Suppl 3), pp. 335-338.

[4] Hubert Chassaigne, Ryszard Lobinski. (1999). “Detection of artifacts and identification in reversed-phase HPLC of metallothionein by electro spray mass spectrometry”, Talanta, 48, pp. 109-118.

[5] Manu Soto, Ionan Marigomer, Ibon Cancio. (1996). “Biological aspects of metal accumulation and storage”, Cell Biology and Histology Lab, Faculty of Science and Technology. University of the Basque Country. POB. 644 E- 48080 Bilbo, Basque Country.

[6] Perceval. O., B. Pinel- Alloul, P. G. C Campbell. (2002). “Cadmium accumulation and metallothionein synthesis in freshwater bivalves (Pyganodon grandis), relative influence of the metal exposure gradient versus immunological variability”, Environmental Pollution, 118, pp. 5-17.

[7] Waldock. M. J., Peter Calow. (1994). Bioaccumulation processes, Handbook of Toxicology vol 1, pp. 379.

[8] Monisha Jaishankar, Tenzin Tseten, Naresh Anbalagan, Blessy B. Mathew, and Krishnamurthy N. Beeregowda. (2014). “Toxicity, mechanism and health effects of some heavy metals.” Published online 2014 Nov 15. Interdiscip Toxicol doi: 10.2478/intox-2014-0009 PMCID: PMC4427717 PMID: 26109881.

[9] Anna Jakimska, Piotr Konieczka, Krzysztof Skóra, Jacek Namieśnik. “ Bioaccumulation of Metals in Tissues of Marine Animals, Part I: the Role and Impact of Heavy Metals on Organisms”. Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, G. Narutowicza 11/12, 80-233 Gdańsk, Poland 2Marine Station Institute of Oceanography in Hel (G215).

[10] Hazrat Ali, Ezzat Khan, and Ikram Ilahi. (2015). “Environmental Chemistry and Ecotoxicology of Hazardous Heavy Metals: Environmental Persistence, Toxicity, and Bioaccumulation” Volume 2019 |Article ID 6730305 | 14 pages PMID: 26690422.

[11] Arif Tasleem Jan, Mudsser Azam, Kehkashan Siddiqui, Arif Ali, Inho Choi, and Qazi Mohd. Rizwanul Haq. (2015). “Heavy Metals and Human Health: Mechanistic Insight into Toxicity and Counter Defense System of Antioxidants.” Reinhard Dallinger, Academic Editor. Int J Mol Sci. 2015 Dec; 16(12): 29592-29630. Published online 2015 Dec 10. doi: 10.3390/ijms161226183.

[12] Gintare Sauliute and Svecevicius. (2015). “Heavy metal interactions during accumulation via direct route in fish”. Nature research Centre, Institute of Ecology LT-08412 Vinius-21 Lithuania Article (PDF Available) January 2015 with 501 Reads.

How to cite this paper

Bioaccumulation, Depuration of Heavy Metals (As, Cd, Pb) and Metabolism of These Metals in Body of Mussels (Maretrix Lyrata) During 20 Days in Artificial Media of Culture

How to cite this paper: Pham Kim Phuong, Nguyễn thị Dung, Chu Pham Ngoc Son, Lưu Duẩn. (2020) Bioaccumulation, Depuration of Heavy Metals (As, Cd, Pb) and Metabolism of These Metals in Body of Mussels (Maretrix Lyrata) During 20 Days in Artificial Media of Culture. International Journal of the Science of Food and Agriculture, 4(3), 237-243.