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International Journal of Clinical and Experimental Medicine Research

ISSN Print: 2575-7989 Downloads: 225449 Total View: 2529975
Frequency: quarterly ISSN Online: 2575-7970 CODEN: IJCEMH
Email: ijcemr@hillpublisher.com
Article Open Access http://dx.doi.org/10.26855/ijcemr.2020.10.005

Role of MicroRNA-98 in targeting IRS-2, Regulation of Insulin Signaling and Glucose Uptake in the Liver after Bariatric Surgery

Lei Sun 1, Yong Wang 2,*

1 Fuxin Center Hospital, China.

2 Department of Bariatric Surgery, The Fourth Affiliated Hospital of China Medical University, China.

*Corresponding author: Yong Wang

Published: August 24,2020

Abstract

Background: Dysregulation of microRNA expression in various tissues and body fluids has been associated with several diseases, including Type 2 Diabetes Mellitus. In this study, we measured the difference in the expression of microRNA in liver tissue of db/+ and db/db mice. And then, we hypothesized that bariatric surgery could change expression of a certain microRNA which may contribute to the remission of insulin resistance. Methods: Db/+ and db/db mice were used to produce bariatric surgery models, then the expression of microRNA in different groups were measured after surgery. With Luciferase assay, we confirmed that microRNA-98 which may be related to the insulin resistance directly targets IRS-2 3’UTR. MicroRNA-98 levels were measured by real-time PCR in db/db mice. Insulin signaling was detected with immunoblotting and glucose uptake. Involvement of IRS2 was studied with microRNA mimics and inhibitor. The role of microRNA-98 in vivo was ex-plored with microRNA antagomir. Results: MicroRNA-98 expression in he-patocyte of db/db mice was decreased after bariatric surgery as time goes on. We demonstrated that microRNA-98 increased in the liver of db/db mice compairing with db/+ mice (1.85±0.82 vs 0.51±0.27 P=0.037). Overexpres-sion of microRNA-98 decreases IRS2 protein levels (0.73±0.05 relative gene expression unit(a.u) vs 0.52±0.04 a.u P=0.003 & 0.26±0.03 a.u P<0.001) and activity and inhibition of microRNA-98 by antisense oligonucleotides increases IRS2 protein levels (1±0.09 a.u vs 1.28±0.082 a.u P=0.011 & 1.52± 0.05 a.u P=0.001) and activity. Meanwhile, microRNA-98 can affect the uptake of glucose in insulin-resistance cultured hepG2 cells (21.79±2.01mM vs 30.51±1.19 mM P = 0.002 & 28.62±1.15 mM P=0.004 & 15.09±1.87 mM P = 0.009). In addition, inhibition of microRNA-98 by antagomir improves the level of blood glucose in db/db mice through mediating expression of IRS2.

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How to cite this paper

Role of MicroRNA-98 in targeting IRS-2, Regulation of Insulin Signaling and Glucose Uptake in the Liver after Bariatric Surgery

How to cite this paper: Lei Sun, Yong Wang. (2020) Role of MicroRNA-98 in targeting IRS-2, Regulation of Insulin Signaling and Glucose Uptake in the Liver after Bariatric Surgery. International Journal of Clinical and Experimental Medicine Research, 4(4), 145-156.

DOI: http://dx.doi.org/10.26855/ijcemr.2020.10.005