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International Journal of Food Science and Agriculture

ISSN Online: 2578-3475 Downloads: 242199 Total View: 3177763
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Article Open Access http://dx.doi.org/10.26855/ijfsa.2021.03.019

Development of Seed Vigour Testing Method Using Single Count of Radicle Emergence for True Seed of Shallot (Allium ascalonicum B.)

Blair Moses Kamanga1, Endah R. Palupi1,*, Eny Widajati2, Satriyas Ilyas2

1Graduate School (Seed Science and Technology Study Program), Bogor Agricultural University, Jl. Agatis, Dramaga Campus, Bogor, Indonesia.

2Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University (Bogor Agricultural University), Dramaga Campus, Bogor 16680, Indonesia.

*Corresponding author: Endah R. Palupi

Published: March 11,2021

Abstract

Seed vigour is one of the seed parameters that determines seed quality for successful crop production. An experiment was conducted at Bogor Agricultural University to develop a seed vigour testing method for the true seed of shallot (TSS) using a single count of radicle emergence (RE) that can be used to estimate germination percentage of normal seedlings in the laboratory and field emergence. Nine artificially made seed lots of TSS were used in this experiment which were germinated at two different temperatures i.e., at 20±1℃ and at 25±1℃ (room temperature). The experiments were arranged in a completely randomized design (CRD) with a repeated measurement, having four replicates. The results showed that a single count of RE at 72 and 68 hours germinated at 20±1℃ and 25±1℃ respectively, were able to predict germination percentage in the laboratory 12 days after sowing and field emergence 14 days after planting. Regression and correlation analysis values of RE were predictive to germination percentage with r=0.891 and R2=0.951 (p<0.01) for seed lots germinated at 20±1℃ and r=0.924 and R2=0.875 (p=<0.01) for seed lots germinated at 25±1℃. Further-more, RE was predictive to field emergence (FE %) with r=0.953 and R2=0.909 (p<0.01) and r=0.865 and R2=0.732 (p<0.01) for seed lots germinated at 20±1℃ and 25±1℃ respectively. A single count of RE correlated well with other vigour tests at 68 hours and 72 hours for seed lots germinated at 25±1℃ and/or 20±1℃ respectively, then it can be used as one of the methods for seed vigour test for TSS.

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How to cite this paper

Development of Seed Vigour Testing Method Using Single Count of Radicle Emergence for True Seed of Shallot (Allium ascalonicum B.)

How to cite this paper: Blair Moses Kamanga, Endah R. Palupi, Eny Widajati, Satriyas Ilyas. (2021) Development of Seed Vigour Testing Method Using Single Count of Radicle Emergence for True Seed of Shallot (Allium ascalonicum B.). International Journal of Food Science and Agriculture, 5(1), 152-162.

DOI: http://dx.doi.org/10.26855/ijfsa.2021.03.019

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