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International Journal of Clinical and Experimental Medicine Research

ISSN Online: 2575-7970 ISSN Print: 2575-7989 CODEN: IJCEMH
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ArticleOpen Access http://dx.doi.org/10.26855/ijcemr.2026.01.012

Molecular Mechanism of Long Chain Non-coding RNA LncRNA H19/Mirna-29A/PRMT5 Axis Expression Promoting Gastric Cancer Cell Invasion and Proliferation

Haibin Ni1, Qiang Hu1, Xiaohua Chen2, Fenglang Xiao2, Meihua Dai2,*

1Department of Gastrointestinal Pancreatic Surgery, Tongde Hospital of Zhejiang Province, Hangzhou 310013, Zhejiang, China.

2Wuxing District People's Hospital of Huzhou City (Wuxing District Maternal and Child Health Hospital of Huzhou City), Huzhou 313000, Zhejiang, China.

*Corresponding author: Meihua Dai

Zhejiang Province Public Welfare Project/Analysis and Testing Project, Analysis of the maintenance effect of CAFs extracellular derived lncRNA H19 on gastric cancer stem cells through miRNA-29a-Sirt1 mediated Sox2 deacetylation (Project approval fund number: LGC22H160003).
Published: January 25,2026

Abstract

Background: In recent years, more and more literature reports have proved the function of long non-coding RNA (lncRNA) in human diseases. LncRNA H19 is a newly defined lncRNA that has been reported as a carcinogenic gene for multiple myeloma. However, the role of lncRNA H19 in gastric cancer is still uncertain. Materials and Methods: We used real-time PCR analysis to detect the expression level of lncRNA H19 in human gastric cancer tissues and cell lines. In addition, we confirmed the distribution of lncRNA H19 in gastric cancer cells and the inhibition of lncRNA H19 expression with siRNA. CCK-8, colony formation assay, and apoptosis analysis were used to investigate the function of lncRNA H19 in the progression of gastric cancer. Then, we use bioinformatics predictions and subsequent experiments to reveal potential molecular mechanisms. Result: We found that lncRNA H19 was upregulated in gastric cancer tissues and cell lines. LncRNA H19 is mainly located in the cytoplasm of gastric cancer cells. Knocking out lncRNA H19 leads to reduced proliferation and increased apoptosis of gastric cancer cells. Mechanically, our data suggest the presence of the lncRNA H19/miRNA-29A/PRMT5 regulatory axis in gastric cancer. LncRNA H19 can activate PRMT5 expression and promote gastric cancer progression by acting as a sponge for miRNA-29A. Conclusion: The expression of lncRNA H19 is elevated in human gastric cancer, promoting growth and inhibiting gastric cancer cell apoptosis. The regulatory cascade lncRNA H19/miRNA-29A/PRMT5 plays a crucial role in the progression of gastric cancer.

Keywords

LncRNA H19; miR-16; PRMT5; Gastric Cancer

References

[1] Siegel RL, Miller KD, Jemal A et al. Cancer statistics, 2019. CA Cancer J Clin. 2023;69:17-29.

[2] Xiao Y, Yu D. Tumor microenvironment as a therapeutic target in cancer. Pharmacol Ther. 2024;56:847-856.

[3] Azadi S, Tafazzoli Shadpour M. The microenvironment and cytoskeletal remodeling in tumor cell invasion. Int Rev Cell Mol Biol. 2022;35:257-289.

[4] Farc O, Cristea V. An overview of the tumor microenvironment, from cells to complex networks (Review). Exp Ther Med. 2021;36:247-254.

[5] Curtis LT, Sebens S, Frieboes HB. Modeling of tumor response to macrophage and T lymphocyte interactions in the liver meta-static microenvironment. Cancer Immunol Immunother. 2023;49:855-864.

[6] Wolf-Dennen K, Kleinerman ES. Exosomes: Dynamic mediators of extracellular communication in the tumor microenvironment. Adv Exp Med Biol. 2024;59:127-136.

[7] Carvalho AS, Baeta H, Silva BC et al. Extra-cellular vesicles carry proteome of cancer hallmarks. Front Biosci (Landmark Ed). 2020;25:398-436.

[8] Wang J, Zhang X, Chen W et al. Regulatory roles of long noncoding RNAs implicated in cancer hallmarks. Int J Cancer. 2023;56:947-958.

[9] Wang W, Han Y, Jo HA et al. Non-coding RNAs shuttled via exosomes reshape the hypoxic tumor microenvironment. J Hematol Oncol. 2020;42:348-352.

[10] Zhang G, Pian C, Chen Z et al. Identification of cancer-related miRNA-lncRNA biomarkers using a basic miRNA-lncRNA network. PLoS One. 2023;48:947-956.

[11] Tong Y, Ru B, Zhang J. miRNACancerMAP: an integrative web server inferring miRNA regulation network for cancer. Bioinformatics. 2018;39:847-853.

[12] Vergara IA, Erho N, Triche TJ et al. Genomic "Dark Matter" in Prostate Cancer: Exploring the clinical utility of ncRNA as biomarkers. Front Genet. 2022;37:849-858.

[13] Sotgia F, Fiorillo M, Lisanti MP. Hallmarks of the cancer cell of origin: Comparisons with "energetic" cancer stem cells (e-CSCs). Aging (Albany NY). 2019;45:747-755.

[14] Sancho P, Barneda D, Heeschen C. Hallmarks of cancer stem cell metabolism. Br J Cancer. 2022;59:847-856.

[15] Singh A, Settleman J. EMT, cancer stem cells and drug resistance: an emerging axis of evil in the war on cancer. Oncogene. 2020;29:441-450.

[16] Zeng H, Ji J, Song X et al. Stemness related genes revealed by network analysis associated with tumor immune microenvironment and the clinical outcome in lung adenocarcinoma. Front Genet. 2020;34:389-397.

[17] Sarkar FH, Li Y, Wang Z et al. Pancreatic cancer stem cells and EMT in drug resistance and metastasis. Minerva Chir. 2019;64:489-500.

[18] Hu C, Li M, Guo T et al. Anti-metastasis activity of curcumin against breast cancer via the inhibition of stem cell-like properties and EMT. Phytomedicine. 2019;58:775-784.

[19] Zhang C, Wang F, Zuo Q et al. Cped1 promotes chicken SSCs formation with the aid of histone acetylation and transcription factor Sox2. Biosci Rep. 2021;38:575-584.

[20] Baltus GA, Kowalski MP, Zhai H et al. Acetylation of Sox2 induces its nuclear export in embryonic stem cells. Stem Cells. 2019;37:175-184.

[21] Tsai CY, Ko HJ, Chiou SJ et al. NBM-BMX, an HDAC8 Inhibitor, Overcomes Temozolomide Resistance in Glioblastoma Multiforme by Downregulating the beta-Catenin/c-Myc/SOX2 Pathway and Upregulating p53-Mediated MGMT Inhibition. Int J Mol Sci. 2021;39:226-229.

[22] Terasaki K, Gen Y, Iwai N et al. SOX2 enhances cell survival and induces resistance to apoptosis under serum starvation conditions through the AKT/GSK-3beta signaling pathway in esophageal squamous cell carcinoma. Oncol Lett. 2021;21:26-29.

[23] Mu P, Zhang Z, Benelli M et al. SOX2 promotes lineage plasticity and antiandrogen resistance in TP53- and RB1-deficient pros-tate cancer. Science. 2017;355:84-88.

How to cite this paper

Molecular Mechanism of Long Chain Non-coding RNA LncRNA H19/Mirna-29A/PRMT5 Axis Expression Promoting Gastric Cancer Cell Invasion and Proliferation

How to cite this paper: Haibin Ni, Qiang Hu, Xiaohua Chen, Fenglang Xiao, Meihua Dai. (2026) Molecular Mechanism of Long Chain Non-coding RNA LncRNA H19/Mirna-29A/PRMT5 Axis Expression Promoting Gastric Cancer Cell Invasion and Proliferation. International Journal of Clinical and Experimental Medicine Research, 10(1), 54-62.

DOI: http://dx.doi.org/10.26855/ijcemr.2026.01.012

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