Application and quality control of molecular biology test methods for hepatitis B

Abstract

With the advancement of the times, people's quality of life has gradually improved, and health issues have gradually been valued, and various intractable diseases are also looking for solutions. Hepatitis B is mainly caused by a virus, and the main detection method of the virus is ELISA. The biological detection methods of hepatitis B molecules mainly include common chain enzyme polymerization reaction method and real-time quantitative PCR method. Among these methods, the fluorescent quantitative PCR method for hepatitis B molecules is more accurate, more sensitive and faster. The advantage of being able to quantify is therefore an ideal method for testing hepatitis B virus. This article mainly describes several methods for detecting hepatitis B, focusing on the advantages and applications of fluorescence quantitative PCR, and then analyzing the key points of biological testing quality control of hepatitis B molecules, such as sensitivity, specificity and preparation methods.