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DOI:http://dx.doi.org/10.26855/ijfsa.2021.12.020

Evaluation of Loop-Mediated Isothermal Amplification for the Detection of Salmonella from Poultry Matrices

Date: December 10,2021 |Hits: 1161 Download PDF How to cite this paper

Raj Rajagopal1,*, Gabriela Lopez-Velasco1, John M. David1, Melissa Sisemore2, Jamie Goseland2

13M Food Safety, 3M Company, St. Paul, MN 55144, USA.

2WBA Analytical Laboratories, Inc., Springdale, AR 72762, USA.

*Corresponding author: Raj Rajagopal

Abstract

Loop-mediated isothermal amplification (LAMP) has emerged as an alternative to PCR based methods for detection of food-borne pathogens, offering simple, easy to use, detection technology with high speed, efficiency, sensitivity and specificity. The performance of LAMP-bioluminescent assay as an alternative method for the detection of Salmonella in primary production samples, poultry rinses and raw poultry products compared to PCR was evaluated. After enrichment, boot swabs from poultry farms, carcass rinses and raw poultry products were tested by a LAMP-bioluminescent and a PCR assay. The LAMP-bioluminescent assay was able to detect Salmonella in the various matrices tested and had higher or equivalent sensitivity and specificity to the PCR method used. No significant difference (95% confidence interval) was found between the LAMP and PCR method as determined by probability of detection analysis. The Salmonella LAMP-bioluminescent assay enabled reliable and rapid detection of Salmonella in variety of poultry matrices and is an acceptable alternative to the PCR method.

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How to cite this paper

Evaluation of Loop-Mediated Isothermal Amplification for the Detection of Salmonella from Poultry Matrices

How to cite this paper: Raj Rajagopal, Gabriela Lopez-Velasco, John M. David, Melissa Sisemore, Jamie Goseland. (2021) Evaluation of Loop-Mediated Isothermal Amplification for the Detection of Salmonella from Poultry MatricesInternational Journal of Food Science and Agriculture5(4), 717-727.

DOI: http://dx.doi.org/10.26855/ijfsa.2021.12.020

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